The Image-Guided Software interfaces with virtually all ultrasound equipment to create and guide a transperineal biopsy plan for the patient. Using the ultrasound interface, the physician first creates a 3D image of the patient’s gland. The Image-Guided Software then creates a biopsy plan and generates continuous 3D images that inform the physician where the first and subsequent needles are placed, digitally tracking the inserted biopsy needles. As samples are taken, the Image-Guided Software creates a comprehensive patient file that numbers the samples, along with their precise location and orientation. It then links each one to a corresponding number on the Biopsy Device and Integrated Pathology System. Upon receipt of the pathology report, the physician then enters the location and aggressiveness of each tumor into the Image-Guided Software for follow-up treatment.